safety

Potential risk score of uploaded strain

ARGs	VFs	PGs	PRS
1	0	2	2.24

PRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BBHNNFHC_35	1298	3217	tet(M)	ARO:3000186	tet(M)	98.90	100.00	AB039845.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BBHNNFHC_35	1298	3217	tet(M)	Doxycycline, Tetracycline, Minocycline	99.69	100.00	X75073

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BBHNNFHC_35	1301	3217	tet(M)	tetracycline resistance ribosomal protection protein Tet(M)	BLASTX	99.69	100.00	WP_011117014.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BBHNNFHC_24	4144	4848	WalR	9.56e-113	transcriptional regulatory protein	83.40	100.00	EPH95667
BBHNNFHC_13	50571	50374	CspR	4.01e-33	cold shock protein	87.88	100.00	AAO82613

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BBHNNFHC_1	0.0	1.0	0.0
BBHNNFHC_2	0.0	1.0	0.0
BBHNNFHC_3	1.0	0.0	1.0
BBHNNFHC_4	0.0	1.0	0.0
BBHNNFHC_5	1.0	0.0	1.0
BBHNNFHC_6	1.0	0.0	1.0
BBHNNFHC_7	0.0	1.0	0.0
BBHNNFHC_8	1.0	0.0	1.0
BBHNNFHC_9	1.0	0.0	1.0
BBHNNFHC_10	1.0	0.0	1.0
BBHNNFHC_11	1.0	0.0	1.0
BBHNNFHC_13	0.0	1.0	0.0
BBHNNFHC_24	0.0	1.0	0.0
BBHNNFHC_35	0.0	1.0	0.0
BBHNNFHC_46	0.0	1.0	0.0
BBHNNFHC_57	0.0	1.0	0.0
BBHNNFHC_68	0.0	1.0	0.0
BBHNNFHC_79	0.0	1.0	0.0
BBHNNFHC_90	0.0	1.0	0.0
BBHNNFHC_98	0.0	1.0	0.0
BBHNNFHC_99	0.0	1.0	0.0
BBHNNFHC_100	0.0	1.0	0.0
BBHNNFHC_101	0.0	1.0	0.0
BBHNNFHC_102	0.0	1.0	0.0
BBHNNFHC_103	0.0	1.0	0.0
BBHNNFHC_104	0.0	1.0	0.0
BBHNNFHC_105	0.0	1.0	0.0
BBHNNFHC_106	0.0	1.0	0.0
BBHNNFHC_107	0.0	1.0	0.0
BBHNNFHC_108	0.0	1.0	0.0
BBHNNFHC_109	0.0	1.0	0.0
BBHNNFHC_110	0.0	1.0	0.0
BBHNNFHC_111	0.0	1.0	0.0
BBHNNFHC_112	0.0	1.0	0.0
BBHNNFHC_113	0.0	1.0	0.0
BBHNNFHC_114	0.0	1.0	0.0
BBHNNFHC_115	0.0	1.0	0.0
BBHNNFHC_116	0.0	1.0	0.0
BBHNNFHC_117	0.0	1.0	0.0
BBHNNFHC_118	0.0	1.0	0.0
BBHNNFHC_119	0.0	1.0	0.0
BBHNNFHC_120	0.0	1.0	0.0
BBHNNFHC_121	0.0	1.0	0.0
BBHNNFHC_122	0.0	1.0	0.0
BBHNNFHC_123	0.0	1.0	0.0
BBHNNFHC_124	0.0	1.0	0.0
BBHNNFHC_125	0.0	1.0	0.0
BBHNNFHC_126	0.0	1.0	0.0
BBHNNFHC_127	0.0	1.0	0.0
BBHNNFHC_128	0.0	1.0	0.0
BBHNNFHC_129	0.0	1.0	0.0
BBHNNFHC_130	0.0	1.0	0.0
BBHNNFHC_131	0.0	1.0	0.0
BBHNNFHC_132	0.0	1.0	0.0
BBHNNFHC_133	0.0	1.0	0.0
BBHNNFHC_134	0.0	1.0	0.0
BBHNNFHC_135	0.0	1.0	0.0
BBHNNFHC_136	0.0	1.0	0.0
BBHNNFHC_137	0.0	1.0	0.0
BBHNNFHC_138	0.0	1.0	0.0
BBHNNFHC_139	0.0	1.0	0.0
BBHNNFHC_140	0.0	1.0	0.0
BBHNNFHC_141	0.0	1.0	0.0
BBHNNFHC_142	0.0	1.0	0.0
BBHNNFHC_143	0.0	1.0	0.0
BBHNNFHC_144	0.0	1.0	0.0
BBHNNFHC_145	0.0	1.0	0.0
BBHNNFHC_146	0.0	1.0	0.0
BBHNNFHC_147	0.0	1.0	0.0
BBHNNFHC_148	0.0	1.0	0.0
BBHNNFHC_149	0.0	1.0	0.0
BBHNNFHC_150	0.0	1.0	0.0
BBHNNFHC_151	0.0	1.0	0.0
BBHNNFHC_152	1.0	0.0	1.0
BBHNNFHC_153	0.0	1.0	0.0
BBHNNFHC_154	0.0	1.0	0.0
BBHNNFHC_155	1.0	0.0	1.0
BBHNNFHC_156	1.0	0.0	1.0
BBHNNFHC_157	1.0	0.0	1.0
BBHNNFHC_158	0.0	1.0	0.0
BBHNNFHC_159	1.0	0.0	1.0
BBHNNFHC_160	1.0	0.0	1.0
BBHNNFHC_161	0.0	1.0	0.0
BBHNNFHC_162	0.0	1.0	0.0
BBHNNFHC_163	0.0	1.0	0.0
BBHNNFHC_164	0.0	1.0	0.0
BBHNNFHC_165	0.0	1.0	0.0
BBHNNFHC_166	0.0	1.0	0.0
BBHNNFHC_167	0.0	1.0	0.0
BBHNNFHC_168	0.0	1.0	0.0
BBHNNFHC_169	0.0	1.0	0.0
BBHNNFHC_170	0.0	1.0	0.0
BBHNNFHC_171	1.0	0.0	1.0
BBHNNFHC_172	1.0	0.0	1.0
BBHNNFHC_173	1.0	0.0	1.0
BBHNNFHC_174	0.0	1.0	0.0
BBHNNFHC_175	0.0	1.0	0.0
BBHNNFHC_176	0.0	1.0	0.0
BBHNNFHC_177	0.0	1.0	0.0
BBHNNFHC_179	0.0	1.0	0.0
BBHNNFHC_180	1.0	0.0	1.0
BBHNNFHC_181	1.0	0.0	1.0
BBHNNFHC_182	1.0	0.0	1.0
BBHNNFHC_183	1.0	0.0	1.0
BBHNNFHC_184	1.0	0.0	1.0
BBHNNFHC_186	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BBHNNFHC_13	2	27998	False	Siphoviridae	VOG4599, VOG4605, VOG4545, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG1329, VOG4555, VOG4564, VOG0720, VOG4544, VOG0796, VOG4549, VOG10904, VOG4570, VOG0198, VOG3643, VOG3643, VOG6131, VOG2820, VOG9759, VOG9741, VOG4693, VOG6623, VOG9860, VOG9667, VOG11003, VOG0226, VOG4566, VOG0186, VOG1309
BBHNNFHC_165	17027	29661	False	Siphoviridae	VOG1581, VOG4632, VOG4609, VOG4841, VOG4581, VOG4544, VOG5051, VOG4556, VOG4568, VOG0204, VOG4589